P-061 In situ microfluidics of fluidic walls: a novel deviceless and cost-effective approach for sperm selection in the same ICSI-dish

Abstract Study question Is our novel deviceless method based on in-situ microfluidics a valuable strategy to select suitable sperm for ICSI? Summary answer The protocol allows selecting ICSI within 15 minutes, in the same dish and disregarding fungible supplies, centrifugation washing steps. What is known already Microfluidics an innovative operation ART which integrates guidance biomimicry during vitro selection process. In contrast other preparation methods such as Wash-Swim-Up Density- Gradients-Centrifugation, disregards steps along procedure. addition, are time-efficient reduce risks associated with handling, gamete mix-up ROS production. However, devices costly likely fail motile dispermic samples. With aim of outlining application ART, we conceived lab-on-a-chip approach by handmade fluidic walls. design, size, duration circuit conforms Laplace hydrostatic pressure laws mechanisms rheotaxis boundary-following behavior. System’s efficacy volume differences, distances between microdoplets, their arrangement. It divides into Section 1 (dispersion seminal plasma removal) 2 (sperm sorting response positive rheotaxis). system was adjusted recover at least 20 selected progressive less than minutes. Participants/materials, setting, We prepared conventional IVF polystyrene round (15 mm diameter) using MOPS buffer, PVP mineral oil only. verified flow-driven dynamics registering horizontal displacement (direction) flow velocity (µm/s) eosin-Y methylene blue dyes. confirmed removal PSA assay. proof-of-concept analysis performed fresh semen samples from 150 patients (Caucasian; mean age: 33,53±8,19). Main results role chance Our design includes three main points allow separation: (initial), (rebalancing), 3 (final) total distance 1,5 cm 3. assures continuous microfluidic flow, two opposed directions (from point 2). assay showed significant decrease levels >200000 ng/ml sample undeterminable (<0,0091 ng/ml) To assess utility number used following characteristics: 3,6 (1,0-7,6) ml, concentration 68 (0,29-150) ×106/ml, motility 24 (2-41) % morphology 6 (1-15) normal. minimum maximum times recovery were recorded 5 35 respectively. Normozoospermic required lower compared (p < 0,001), although according median, × 106/ml need ten minutes time. Sperm parameters: concentration, inversely correlated time 0,0001), being parameter lesser influence outcome. Limitations, reasons caution present depends free-hand preparation. Although use templates training reduces inter intra-operator variability, ready-to-use surface would benefit operability. Non-inferiority studies compare currently ones (Wash-Swim-Up or Density-Gradients-Centrifugation) before clinical implementation. Wider implications findings methodology streamlines This represents paradigm shift preparation, since it dispenses centrifugation, plasticware. can be implemented clinic near-term, particularly due its simplicity. Trial registration na